Chengdu Pukang is one of the most professional manufacturers for short peptide with all completed pharmacological studies, and the impurity profiles have been systematically studied and controlled, can reach 100-kilogram-scale production. Using certain short peptide fragments as starting materials is one of the ways to chemically synthesize peptides. Compared with protecting amino acids, using short peptide fragments as starting materials can shorten the production cycle, effectively control some specific impurities, and is an effective means to deal with difficult-to-synthesize sequences. Pukang has established a short peptide platform to assist the development and industrialization of new peptide drugs, including the following categories: Semaglutide short peptide, Tirzepatide short peptide, pseudodipeptide, and other customized short peptides. The short peptides in the Pukang platform have all completed pharmacological studies in accordance with the requirements of ICH M4, and the impurity profiles have been systematically studied and controlled, and 100-kilogram-scale production has been realized. Pseudodipeptide series are the key research products of Pukang short peptide platform. The Pro-like structure formed in pseudodipeptide cannot form hydrogen bond, which can effectively inhibit the formation of β-fold, reduce aggregation and increase solubility, so that many difficult-to-synthesize sequences are no longer a problem. In peptide drugs, amino acid linkage methods mainly include biological fermentation and solid phase synthesis. In solid-phase synthesis, amino acids are connected one by one, with a long process and a long time cycle. We have developed dipeptide compounds that can cut the synthesis cycle in half and greatly improve product purity. Pseudo-dipeptide consists of two amino acid residues joined in the form of acylthione and enoate. First, pseudo-dipeptides have high stability and biological activity. They are able to maintain their original chemical structure and maintain a certain stability under various conditions in the body.Secondly, pseudo-dipeptides can mimic the structure and function of natural peptides and have similar biological activities to natural peptides. Therefore, they can be used as candidates for drug molecules for the development of antibody drugs, peptide drugs, and other specialty drugs. Finally, pseudo-dipeptides can be used in the field of chemical synthesis to construct polypeptide chains and synthesize various organic molecules. By properly designing and selecting the structure and reaction conditions of pseudo-dipeptides, the selectivity and efficiency of chemical reactions can be effectively controlled, to realize the synthesis and functional design of complex organic molecules.